By Baxter G.P., Hartmann M.L.

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Extra info for A Revision of the Atomic Weight of Cadmium (1914)(en)(4s)

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1). In the experiments described below, the sites of breakage were localized using the ends of the YAC as a reference point. To that purpose, the mixture of the molecules resulting from a partial digestion of the YAC was fractionated by agarose gel electrophoresis and transferred to a positively charged nylon membrane. The hybridization of the membrane with a 32P-labeled probe, specific to one end of the YAC, revealed the unbroken YAC DNA molecules as well as their products of breakage. As a probe, we used a fragment of pBR322 (see Note 2).

And Simchen, G. (1996) Double-strand breaks on YACs during yeast meiosis may reflect meiotic recombination in the human genome. Nat. Genet. 13, 481–484. 2. , and Simchen, G. (1994) A versatile method for efficient YAC transfer between any two strains. Genomics 22, 108–117. 3. Spencer, F. and Simchen, G. (1996) Transfer of YAC clones to new yeast hosts. Methods Mol. Biol. 54, 239–252. 50 Ira et al. 4. Ira, G. D. Thesis. M, Kopernik University, Torun, Poland. 5. , and Filipski, J. (1998) Meiotic double-strand breaks in yeast artificial chromosomes containing human DNA.

2) offers the advantage of simplicity and its use on fresh slides. Standard RBA-banded karyotypes for cattle, sheep, goat, and river buffalo are available (3,4,10). 6. RBG banding (Fig. 3) offers (1) higher banding pattern resolution than RBA banding (more bands compared with those achieved with RBA banding), (2) the possibility of treating slides and working on them later, and (3) keeping slides for years after staining. 7. R banding is the best banding technique to be applied routinely on domestic animal chromosomes in clinical, evolutionary, and molecular (FISH) cytogenetics (9,16–23).

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A Revision of the Atomic Weight of Cadmium (1914)(en)(4s) by Baxter G.P., Hartmann M.L.

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